Plasmodium berghei: effect of protease inhibitors during gametogenesis and early zygote development |
| |
Authors: | Torres Jorge A Rodriguez Mario H Rodriguez Maria C de la Cruz Hernandez-Hernandez Fidel |
| |
Affiliation: | Department of Experimental Parasitology, CINVESTAV-IPN, Av. IPN 2508, Col. San Pedro Zacatenco Delegacion G.A. Madero, Mexico 07360 DF, Mexico. |
| |
Abstract: | Plasmodium berghei: The effect of five protease inhibitors, TPCK, TLCK, PMSF, leupeptin, and 1,10-phenanthroline on in vitro gametogenesis and early zygote development of P. berghei was investigated. PMSF and leupeptin showed no effect. Cysteine/serine protease inhibitors TPCK/TLCK at concentrations of 75 and 100 microM were effective on inhibiting exflagellation center formation, and this effect was reversible with the addition of l-cysteine. Exflagellation center formation was most effectively blocked by 1,10-phenanthroline (1mM), and exflagellation center numbers were restored by the addition of Zn(2+). A reduction of ookinete production was observed when TPCK/TLCK (100 microM) was added at 2h after gametogenesis, but no effect was observed with 1,10-phenanthroline (1mM). Our results suggest that proteolysis is important in both gametocyte activation and sexual development of P. berghei. |
| |
Keywords: | Plasmodium berghei Malaria Parasite Gametogenesis Gametes Zygotes Exflagellation centers Leupeptin PMSF, phenylmethylsulfonyl fluoride TLCK, N-α-p-tosyl- smallcaps" >l-lysine chloromethyl ketone TPCK, smallcaps" >l-1-tosylamide-2-phenylethyl-chloromethyl ketone 1,10-phenanthroline Protease Protease inhibitor |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|