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Formation of DNA-protein cross-links in cultured mammalian cells upon treatment with iron ions |
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| Authors: | Steven A Altman Tomasz H Zastawny Lisa Randers-Eichhorn Marco A Cacciuttolo Steven A Akman Miral Dizdaroglu Govind Rao |
| Institution: | a Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, MD, USA b Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA c Department of Clinical Biochemistry, Medical Academy, Bydgoszcz, Poland d Department of Medical Oncology, City of Hope National Medical Center, Duarte, CA, USA e Medical Biotechnology Center of the Maryland Biotechnology Institute, University of Maryland, Baltimore, MD, USA |
| Abstract: | Formation of DNA-protein crosslinks (DPCs) in mammalian cells upon treatment with iron or copper ions was investigated. Cultured murine hybridoma cells were treated with Fe(II) or Cu(II) ions by addition to the culture medium at various concentrations. Subsequently, chromatin samples were isolated from treated and control cells. Analyses of chromatin samples by gas chromatography/mass spectrometry after hydrolysis and derivatization revealed a significant increase over the background amount of 3-(1,3-dihydro-2,4-dioxopyrimidin-5-yl)-methyl]- |
| Keywords: | DNA damage Hydroxyl radical Oxidative stress Thymine-tyrosine Crosslinks Free radicals |
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