用组织微阵列技术分析鼻咽癌变多阶段组织细胞周期蛋白D1过表达

为了探讨细胞周期蛋白D₁(Cyclin D₁)在鼻咽癌变多阶段病变组织中的蛋白质表达与鼻咽癌变的关系,应用组织微阵列（tissue microarray）技术,采用免疫组化方法检测Cyclin D₁在鼻咽癌旁粘膜上皮单纯性增生/化生(simple hyperplasia/ metaplasia,SH/SM)、异型增生/化生(atypical hyperplasia/metaplasia,AH/AM)和鼻咽癌(nasopharyngeal carcinoma,NPC)的蛋白质表达,阳性表达率分别为30.0%（3/10）、90.0%（18/20）和62.9%（39/62）,其中在异型增生/化生中呈“一过性增高”.表明Cyclin D₁高表达可能为鼻咽癌发生过程中的早期事件;异型增生/化生可能是鼻咽癌变的重要“关卡”.

Tissue Microarray Analysis of Cyclin D1 Gene Overexpression in The Multistage Carcinogenesis of Nasopharyngeal Carcinoma

In order to investigate the relation between Cyclin D 1 protein expression in multistage tissue of pathological changes during the nasopharyngeal carcinogenesis and nasopharyngeal carcinoma(NPC), the simple hyperplasia/ metaplasia, atypical hyperplasia/metaplasia in the nasopharyngeal paracarcinoma mucous epithelium and NPC were studied for Cyclin D 1 expression with immunohistochemical streptavidin peroxidase (SP) method by tissue array techenique. The positive rates were 30 0%(3/10),90 0%(18/20)and 62 9%(39/62)respectively,among which hightening "instantaneously" in the atypical hyperplasia/metaplasia appeared. It is shown high Cyclin D 1 expression may be an early incident in the course of nasopharyngeal carcinogenesis, and that atypical hyperplasia/metaplasia probably be an important "toll gate".