Oncogene alterations in in vitro transformed rat tracheal epithelial cells |
| |
| Institution: | 1. Respiratory Carcinogenesis Group, Carcinogenesis and Metabolism Branch (MD-68), U.S. Environmental Protection Agency, Research Triangle Park, NC 27711 U.S.A.;2. Environmental Health Research and Testing Inc., Research Triangle Park, NC 27711 U.S.A.;1. Specialized Research Unit: Prebiotics and Probiotics for Health, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand;2. Center for Advanced Studies for Agriculture and Food, KU Institute for Advanced Studies, Kasetsart University (CASAF, NRU-KU), Bangkok 10900, Thailand;3. Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Thailand;4. Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERD O-CHE), Bangkok 10900, Thailand;1. Laboratório de Higiene de Alimentos, Departamento de Ciência de Alimentos, Instituto de Ciências e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, Brazil;2. Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Instituto de Ciências e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, Brazil;1. Key Laboratory of Watershed Geographic Sciences, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Department of Geography, Hong Kong Baptist University, Hong Kong, China;1. Center for Innovative Technology on Advanced Forming, School of Mechanical Engineering, Pusan National University, Busan 46241, Republic of Korea;2. School of Material Science and Engineering, Pusan National University, Busan 46241, Republic of Korea;3. Department of Metallurgical and Materials Engineering, Colorado School of Mines, Golden, CO 80401, USA;1. College of Marine Sciences, Shanghai Ocean University, Shanghai, 201306, PR China;2. National Engineering Research Center for Oceanic Fisheries, Shanghai, 201306, PR China;3. Polar Marine Ecosystem Group, Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, PR China;4. Center for Quantitative Fisheries Ecology, Old Dominion University, 800 West 46th St., Norfolk, VA, 23529, USA;6. Institute for Marine and Antarctic Studies, University of Tasmania, Private Bag 129, Hobart, TAS, 7001, Australia;7. CSIRO Oceans and Atmosphere, GPO Box 1538, Hobart, TAS, 7001, Australia |
| |
| Abstract: | 10 derivations of rat tracheal epithelial (RTE) cells, including normal cells, normal primary cultures, 7 tumorigenic cell lines and 1 nontumorigenic cell line transformed in vitro by treatment with 7,12-dimethyl-benza]anthracene (DMBA), benzoa]pyrene (BP) and/or 12-O-tetradecanoylphorbol-13-acetate (TPA) were examined for oncogene alterations. No abnormalities of Ha-ras were seen that were suggestive of amplification, rearrangement or the presence of RFLPs. Analysis of specific-point mutations in Ha-ras using Pst I digestion (codon 12, GGA to GCA) or Ha-ras and Ki-ras using Xba I (codon 61, CAA to CTA) were negative. In one cell line derived by DMBA treatment, changes in the c-myc restriction digest pattern were seen after incubation with Bam HI and Hind III. Northern analysis revealed consistent differences between normal and transformed cells when probed with Ha-ras; c-myc expression was of low intensity, and the expression of Ki-ras could not be detected. Transfection of RTE cell DNAs into NIH/3T3 cells did not result in the appearance of morphologic transformants. The studies suggest that Ha-ras or Ki-ras codon 61 A to T transversions (CAA to CTA) are not associated with the immoral/tumorigenic phenotype in RTE cells transformed by DMBA or TPA, and are in contrast to results reported in some other biological systems. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
