Inhibition of photosynthetic electron transport by amphotericin B |
| |
Authors: | Gerhard Sandmann Peter Böger |
| |
Institution: | Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-7750 Konstanz, Germany. |
| |
Abstract: | By assaying partial reactions of the photosynthetic electron transport system using thylakoids from spinach as well as from the algae Bumilleriopsis, Dunaliella , and Anabaena , it was demonstrated that the polyene antibiotic amphotericin B has no specific effect on plastocyanin. Pretreating spinach and algal thylakoids with this antibiotic decreased photosystem-II as well as photosystem-I activity regardless of whether the membranes contained plastocyanin or cytochrome c-553. Different sensitivity of cell-free electron transport activity against this antibiotic was observed due to the species used. With Dunaliella , the photosystem-II region was inhibited more strongly than photosystem-I, while Bumilleriopsis chloroplasts – although not containing plastocyanin – exhibited a stronger inhibition of the photosystem-I region. Apparently, amphotericin B mainly solubilizes redox compounds that form connecting pools in the photosynthetic electron transport chain. |
| |
Keywords: | Polyene antibiotic plastocyanin thylakoidal cytochrome c-553 algal thylakoids |
|
|