|
|||||
|
|
Decreased expression of the DBC2 gene and its clinicopathological significance in breast cancer: correlation with aberrant DNA methylation |
|
| Authors: | Linlin Han Lin Hou Jinlian Song Dongliang Lin Li Wu Yinlin Ge Zhongliang Ma |
| Institution: | 1. Department of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao, 266021, Shandong, China 2. Department of Laboratory, Women and Children’s Health Care Center of Qingdao, Qingdao, 266011, Shandong, China 3. Department of Pathology, The Affiliated Hospital of Qingdao University Medical College, Qingdao, 266000, Shandong, China 4. Department of Breast Surgery, The Affiliated Hospital of Qingdao University Medical College, Qingdao, 266000, Shandong, China |
| Abstract: | Loss of DBC2 (deleted in breast cancer 2) gene expression is frequent in breast cancer tissues. This can be explained by homozygous deletions or other mutations in a minority of cases but alternative mechanisms need to be investigated. Here, DBC2 expression was significantly suppressed compared with normal breast tissues in breast cancer tissues when analyzed by RT-PCR. Furthermore, DNA methylation on DBC2 was more prevalent in breast tumors than in normal tissues. DBC2 mRNA levels correlated with the degree of DBC2 methylation in breast cancer tissues and in a breast cancer cell line (T47D). Clinico-pathological correlation analysis showed that DBC2 promoter methylation was associated with tumor-node-metastasis stages II and III/IV, lymph node metastasis, p53 mutation, and HER2-positive status. Thus loss of DBC2 expression is caused by abnormal methylation of DBC2 and might have a role in breast cancer development. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |