|
|||||
|
|
Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model |
|
| Authors: | Yaghoub Yazdani Neda Keyhanvar Hamid Reza Kalhor Abbas Rezaei |
| Institution: | 1. Infectious Diseases Research Center and Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran 2. Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran 3. Biochemistry Research Laboratory, Department of Chemistry, Sharif University of Technology, Tehran, Iran 4. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran |
| Abstract: | Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01). |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |