Characterization of the Aminoacylation of Cytoplasmic Transfer Ribonucleic Acids from Germinating Soybeans |
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| Authors: | C. F. TESTER |
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| Affiliation: | Research Chemist, USDA, ARS, Departments of Crop Science and Genetics, North Carolina State University, Raleigh, North Carolina 27607 |
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| Abstract: | Extracts from germinating soybean [Glycine max (L.) Merr.] cotyledons were assayed for aminoacyl-tRNA formation. This esterification reaction depends on several factors, which must be determined and controlled for each system investigated. The reaction was maximum when the Mg: ATP ratio was 12.5 and concentrations were 9 and 0.72 mM, respectively. The optimum temperature for the in vitro reaction was 25°C. In addition, the reaction was inhibited by inorganic pyrophosphate and magnesium could be replaced by spermidine. |
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