| 人硫氧还蛋白在大肠杆菌中的高效表达、纯化及对内毒素血症小鼠的保护作用 |
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| 引用本文： | 曹丽,黄大无,范慧,狄春辉,王应,陈建民,马大龙.人硫氧还蛋白在大肠杆菌中的高效表达、纯化及对内毒素血症小鼠的保护作用[J].中国生物化学与分子生物学报,2002,18(6):81-84. |
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| 作者姓名： | 曹丽 黄大无 范慧 狄春辉 王应 陈建民 马大龙 |
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| 作者单位： | 1. 中国医学科学院中国协和医科大学,药用植物研究所,北京,100094
2. 北京大学人类疾病基因组研究中心,北京,100083 |
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| 基金项目： | 国家重点基础研究发展规划项目 (973 )基金 (No .G2 0 0 0 0 5 690 9)项目~~ |
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| 摘 要： | 为了对人硫氧还蛋白hTRX功能及其在休克治疗前景进行评价 ,将PCR扩增的hTRX基因连入pKPL 4载体 ,转化大肠杆菌pop2 136 .通过两步离子交换柱分离得到纯化的蛋白 ,进一步观察其稳定性及在体外对NFS 6 0和MCF 7细胞的促增殖活性 .结果发现 ,TRX蛋白可明显促进去细胞因子诱导的NFS 6 0细胞增殖和撤血清后MCF 7细胞的增殖 ,并发现外源性TRX可明显对小鼠内毒素血症起保护作用 .
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| 关 键 词： | 人硫氧还蛋白 表达与纯化 内毒素休克 |
| 收稿时间： | 2002-12-20 |
| 修稿时间： | 2002年4月15日 |
The Expression and Purification of Human Thioredoxin from E.coli and Its Protective Effects in a Murine Model of Endotoxemia |
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| CAO Li ,HUANG Da wu ,FAN Hui ,DI Chun\|hui ,WANG Ying ,CHEN Jian\|min ,MA Da\|long.The Expression and Purification of Human Thioredoxin from E.coli and Its Protective Effects in a Murine Model of Endotoxemia[J].Chinese Journal of Biochemistry and Molecular Biology,2002,18(6):81-84. |
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| Authors: | CAO Li HUANG Da wu FAN Hui DI Chun\|hui WANG Ying CHEN Jian\|min MA Da\|long |
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| Institution: | ( 1) Institute of Medicinal Plant,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing\ 100094,China; 2) Peking University Research Center for Human Disease Genomics,Beijing\ 100083,China |
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| Abstract: | To investigate the function of human thioredoxin(hTRX)and its potential value on shock therapy,the hTRX gene was amplified by PCR and cloned into the pKPL 4 vector,then transformed into E.coli pop2136.The recombinant hTRX protein was purified by two steps of chromatography.Its sbability and promotion on the growth of NFS 60 and MCF\|7 cells were investigated in vitro .hTRX significantly promoted the growth of NFS 60 by cytokine withdrawal and MCF\|7 by serum withdrawal,and it showed stability at 4℃ within one month.The studies in vivo showed that TRX could protect mice from lethal endotoxemia. |
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| Keywords: | human thioredoxin expression and purification endotoxic shock |
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