Detection of HSV type-1 and type-2 IgG using an in vitro PCA based homogeneous immunoassay |
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Authors: | Fry Scott R Li Jun de las Heras Rachel McCourt Jennifer A Arel Ece Kachab Edward H Hazell Stuart L Huang Chang-Yi |
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Institution: | Inverness Medical Innovations Australia Pty Ltd (Formerly Panbio Ltd), Department of Research and Development, 532 Seventeen Mile Rocks Road, Sinnamon Park, Brisbane, Qld 4073, Australia |
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Abstract: | Enzyme immunoassays (EIAs) are widely used in the clinical laboratory and research institutes for the detection of biologically relevant analytes. Almost all EIAs are heterogeneous in nature and require multiple steps of process. In contrast, homogeneous immunoassays (HA) offer a simplified one-step approach with a number of potential advantages over contemporary heterogeneous EIAs such as higher throughput and greater clinical utility. Utilizing TEM-1 β-lactamase as a reporter enzyme, we have developed HAs based on in vitro protein fragment complementation (PCA) for the detection of antibodies and potentially be used for antigens or other biomarkers. In this proof-of-principle study we demonstrate the successful in vitro differentiation of anti-herpes simplex virus (HSV) type-1 and type-2 Immunoglobulin G (IgG) in human serum with high sensitivity and specificity. |
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Keywords: | TEM-1 β-lactamase Herpes simplex virus Enzyme immunoassay Protein fragment complementation assay Homogeneous immunoassay |
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