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HGF/SF and menthol increase human glioblastoma cell calcium and migration |
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| Authors: | Wondergem Robert Ecay Tom W Mahieu Frank Owsianik Grzegorz Nilius Bernd |
| Institution: | a Department of Physiology, James H. Quillen College of Medicine, East Tennessee State University, P.O. Box 70,576, Johnson City, TN 37614-1708, USA b Department of Molecular Cell Biology, Division of Physiology, Laboratory of Ion Channel Research, Katholieke Universiteit, B-3000 Leuven, Belgium c GROEP T—Leuven Engineering College, Associatie Katholieke Universiteit, Vesaliusstraat 13, B-3000 Leuven, Belgium |
| Abstract: | This study explored the role of transient receptor potential melastatin 8 ion channels (TRPM8) in mechanisms of human glioblastoma (DBTRG) cell migration. Menthol stimulated influx of Ca2+, membrane current, and migration of DBTRG cells. Effects on Ca2+ and migration were enhanced by pre-treatment with hepatocyte growth factor/scatter factor (HGF/SF). Effects on Ca2+ also were greater in migrating cells compared with non-migrating cells. 2-Aminoethoxydiphenyl borate (2-APB) inhibited all menthol stimulations. RT-PCR and immunoblot analysis showed that DBTRG cells expressed both mRNA and protein for TRPM8 ion channels. Two proteins were evident: one (130-140 kDa) in a plasma membrane-enriched fraction, and a variant (95-100 kDa) in microsome- and plasma membrane-enriched fractions. Thus, TRPM8 plays a role in mechanisms that increase Ca2+]i needed for DBTRG cell migration. |
| Keywords: | Transient receptor potential ion channel TRPM8 Hepatocyte growth factor/scatter factor HGF/SF DBTRG Migration Glioblastoma Calcium |
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