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Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging |
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| Authors: | Fang Yun Shu Dan Xiao Feng Guo Peixuan Qin Peter Z |
| Institution: | a Department of Chemistry, University of Southern California, LJS-251, 840 Downey Way, Los Angeles, CA 90089-0744, USA b Department of Biological Sciences, University of Southern California, LJS-251, 840 Downey Way, Los Angeles, CA 90089-0744, USA c Department of Biomedical Engineering, University of Cincinnati/College of Engineering/College of Medicine, Cincinnati, OH 45221, USA |
| Abstract: | The bacteriophage phi29 DNA packaging motor is a protein/RNA complex that can produce strong force to condense the linear-double-stranded DNA genome into a pre-formed protein capsid. The RNA component, called the packaging RNA (pRNA), utilizes magnesium-dependent inter-molecular base-pairing interactions to form ring-shaped complexes. The pRNA is a class of non-coding RNA, interacting with phi29 motor proteins to enable DNA packaging. Here, we report a two-piece chimeric pRNA construct that is fully competent in interacting with partner pRNA to form ring-shaped complexes, in packaging DNA via the motor, and in assembling infectious phi29 virions in vitro. This is the first example of a fully functional pRNA assembled using two non-covalently interacting fragments. The results support the notion of modular pRNA architecture in the phi29 packaging motor. |
| Keywords: | Packaging RNA Packaging motor DNA packaging Bacteriophage phi29 RNA assembly Non-coding RNA |
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