Guanyl-C8-arylamination of DNA by the ultimate carcinogen of 4-nitroquinoline-1-oxide: a spectrophotometric titration |
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Authors: | S Galiègue-Zouitina B Bailleul M H Loucheux-Lefebvre |
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Affiliation: | Institute de Recherches sur le Cancer et Unité 124 de l''INSERM BP 311, 59020 Lille Cédex, France |
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Abstract: | Native and denatured DNAs and polynucleotides were modified by 4-acetoxyaminoquinoline-1-oxide, the ultimate carcinogen of 4-nitroquinoline-1-oxide (4 NQO). The N-( deoxyguanosin -C8-yl)-4-aminoquinoline-1-oxide adduct, the so-called "dG III," was quantified on the DNA and on poly(dG-dC) in absorption spectroscopy, by using a spectral property of dG III, i.e., the variation of the absorption spectrum as a function of the pH. Using the "free-dG III" absorption reference spectra, a simple graphic determination of the percentage of dG III was established by recording the absorption spectra of the 4-acetoxyaminoquinoline-1-oxide-modified polymers. It was found that the dG III adduct accounts for about 30% of the total modification in the case of native modified DNA and poly(dG-dC) and for about 70% in the case of denatured modified DNA. |
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Keywords: | carcinogen 4-nitroquinoline-1-oxide DNA arylamination absorption spectroscopy addut titration 4 NQO 4-nitroquinoline-1-oxide |
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