Synergistic action of xylanase and mannanase improves the total hydrolysis of softwood |
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Authors: | Várnai Anikó Huikko Laura Pere Jaakko Siika-Aho Matti Viikari Liisa |
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Institution: | a University of Helsinki, Food and Environmental Sciences, P.O. Box 27, 00014 Helsinki, Finland b VTT Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT, Finland |
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Abstract: | The impact of xylan and glucomannan hydrolysis on cellulose hydrolysis was studied on five pretreated softwood substrates with different xylan and glucomannan contents, both varying from 0.2% to 6.9%, using mixtures of purified enzymes.The supplementation of pure cellulase mixture with non-specific endoglucanase TrCel7B and xylanase TrXyn11 enhanced the hydrolysis of all substrates, except the steam pretreated spruce, by more than 50%. The addition of endo-β-mannanase increased the overall hydrolysis yield by 20-25%, liberating significantly more glucose than theoretically present in glucomannan.When supplemented together, xylanolytic and mannanolytic enzymes acted synergistically with cellulases. Moreover, a linear correlation was observed between the hydrolysis of polysaccharides, irrespective of the composition, indicating that glucomannan and xylan form a complex network of polysaccharides around the cellulosic fibres extending throughout the lignocellulosic matrix. Both hemicellulolytic enzymes are crucial as accessory enzymes when designing efficient mixtures for the total hydrolysis of lignocellulosic substrates containing both hemicelluloses. |
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Keywords: | Lignocellulose Enzymatic hydrolysis Mannanase Xylanase Synergism |
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