| 第四双小核草履虫减数分裂产物的退化特征分析 |
| |
| 引用本文： | 高欣,徐川梅,杨仙玉. 第四双小核草履虫减数分裂产物的退化特征分析[J]. 动物学杂志, 2011, 46(3): 89-92 |
| |
| 作者姓名： | 高欣 徐川梅 杨仙玉 |
| |
| 作者单位： | 浙江农林大学资源动物研究所;浙江农林大学林业与生物技术学院; |
| |
| 基金项目： | 浙江农林大学科研发展基金项目(No.2292000030); 浙江省教育厅科研项目(No.Y200804256); 国家自然科学基金项目(No.30670297,31071881) |
| |
| 摘 要： | 通过吖啶橙和Hoechst 33342两种活体荧光染料双染的方法对第四双小核草履虫(Paramecium tetraurelia)接合生殖过程中小核减数分裂产物进行观察,结果发现位于口旁锥外的小核分裂产物呈蓝绿色或黄绿色,表明它们以凋亡的方式发生退化.
|
| 关 键 词： | 第四双小核草履虫 接合生殖 核凋亡 吖啶橙 Hoechst 33342 |
| 收稿时间： | 2010-11-03 |
| 修稿时间： | 2011-01-08 |
Characterization of Meiotic Nuclear Degeneration in Paramecium tetraurelia |
| |
| GAO Xin,XU Chuan-Mei and YANG Xian-Yu. Characterization of Meiotic Nuclear Degeneration in Paramecium tetraurelia[J]. Chinese Journal of Zoology, 2011, 46(3): 89-92 |
| |
| Authors: | GAO Xin XU Chuan-Mei YANG Xian-Yu |
| |
| Affiliation: | Institute of Resource Zoology, Zhejiang Agriculture and Forestry University, Lin'an 311300; School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an 311300, China;School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an 311300, China;Institute of Resource Zoology, Zhejiang Agriculture and Forestry University, Lin'an 311300; School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an 311300, China |
| |
| Abstract: | During the conjugation of Paramecium tetraurelia,one meiotic nucleus which enters the paroral region survives and undergoes mitotic division to form gametic pronuclei,while the remaining seven degenerate.To clarify if these meiotic outcomes degenerate in an apoptotic way,two kinds of vital fluorescence dyes,acridine orange and Hoechst 33342 were used.The observation showed that the degenerating nuclei outside the paroral region stained yellow-orange or greenish which indicates a programmed nuclear degenerat... |
| |
| Keywords: | Paramecium tetraurelia Conjugation Nuclear death Acridine orange Hoechst 33342. |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《动物学杂志》浏览原始摘要信息 |
|
点击此处可从《动物学杂志》下载全文 |
|
