The novel allele (3R) of the VNTR polymorphism in the XRCC5 promoter region dramatically decreases the gene expression |
| |
| Authors: | Mehrdad Rajaei Iraj Saadat Mostafa Saadat |
| |
| Affiliation: | 1. Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing, China;2. Division of Urology, University of Texas Medical, School at Houston, Houston, TX, USA;3. Department of Urology, West China Hospital, Sichuan University, Chengduk, China;4. Department of Pharmacological and Pharmaceutical Sciences, Center for Experimental Therapeutics and PharmacoInformatics, University of Houston, Houston, TX, USA;5. Division of Gastroenterology, Hepatology & Nutrition, Department of Internal, Medicine University of Texas Medical School at Houston, Houston, TX, USA;6. Department of Urology, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA |
| |
| Abstract: | Polymorphism of variable number of tandem repeats (VNTR) in the promoter region of X-ray repair cross-complementing 5 (MIM: 194364, XRCC5; rs6147172) was reported. The aim of the present study is to evaluate the influence of this polymorphism on XRCC5 mRNA levels. Genotypes of XRCC5 VNTR were determined by high resolution of melting analysis (HRMA). The quantitative XRCC5 mRNA expression (compared to ß-actin expression) among 0R/1R, 1R/2R, and 1R/3R genotypes was investigated. There was a negative correlation between the overall number of tandem repeats and XRCC5 expression (r = ?0.965, df = 7, P < 0.001). The mRNA level of XRCC5 decreased as function of number of tandem repeats. The 3R allele of the VNTR polymorphism in the XRCC5 promoter region dramatically decreases the gene expression. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
