Co-culture with endothelial progenitor cells promotes survival,migration, and differentiation of osteoclast precursors |
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| Authors: | Hao Pang Xue-Hui Wu Sheng-Long Fu Fei Luo Ze-Hua Zhang Tian-Yong Hou Zhi-Qiang Li Zheng-Qi Chang Bo Yu Jian-Zhong Xu |
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| Affiliation: | 1. Department of Rheumatology, Kawasaki Medical School, Kurashiki, Japan;2. Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri-Kansas City, MO, USA;3. Department of Nuclear Medicine, Kawasaki Medical School, Kurashiki, Japan |
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| Abstract: | In this study, we report the effect of endothelial progenitor cells (EPCs) on the biological behavior of osteoclast precursors in vitro by establishing an indirect co-culture system of mice EPCs and RAW 264.7 monocyte cells. Results show that the survival, migration, and differentiation of osteoclast precursors were greatly enhanced when co-cultured with EPCs. These phenotypic changes coincide with the upregulation of multiple genes affected cell behavior, including phospho-VEGFR-2, CXCR4, phospho-Smad2/3, phospho-Akt, phospho-ERK1, and phospho-p38 MAPK. The results collectively suggest that EPCs could modulate the survival, migration, and differentiation potential of osteoclast precursors, thus providing new insights in understanding of correlation between angiogenesis and bone homeostasis. |
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