Additional data for a new Theileria sp. from China based on the sequences of ribosomal RNA internal transcribed spacers |
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| Authors: | Junlong Liu Guiquan Guan Zhijie Liu Aihong Liu Miling Ma Qi Bai Hong Yin Jianxun Luo |
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| Affiliation: | 1. Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA;2. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;3. Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA;4. Department of Medical Laboratory Science, University of Benin, Benin City, Nigeria;5. Division of Parasitology, National Institute of Cholera and Enteric Diseases, Kolkata 700010, West Bengal, India;6. Enteric Viruses Research Group, Institute of Primate Research, Karen, Nairobi 00502, Kenya;7. College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun 130062, China;1. Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil;2. Departamento de Patologia, Universidade Estadual do Maranhão, São Luiz, Maranhão, Brazil;1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China;2. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, PR China;3. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China;4. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China;1. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;2. Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil;3. Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;4. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;5. Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil;6. Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas – NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, Brazil;1. School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia;2. Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia 6076, Australia;1. Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China;2. Key Laboratory of Animal Clinical Diagnosis Technology, Ministry of Agriculture, Inter Mongolia Agricultural University, Huhhot 010018, China |
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| Abstract: | Theileria sinensis was recently isolated and named as an independent Theileria species that infects cattle in China. To date, this parasite has been described based on its morphology, transmission and molecular studies, indicating that it should be classified as a distinct species. To test the validity of this taxon, the two internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene were cloned and sequenced from three T. sinensis isolates. The complete ITS sequences were compared with those of other Theileria sp. available in GenBank. Phylogenetic analyses based on sequence data for the complete ITS sequences indicate that T. sinensis lies in a distinct clade that is separate from that of T. buffeli/orientalis and T. annulata. Sequence comparisons indicate that different T. sinensis isolates possess unique sizes of ITS1 and ITS2 as well as species-specific nucleotide sequences. This analysis provides new molecular data to support the classification of T. sinensis as a distinct species from other known Theileria spp. based on ITS sequences. |
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