生物活组织的背向二次谐波成像

光学二次谐波成像技术由于具有三维高分辨率、不需要荧光标记、对生物样品的杀伤效应小等特点，在生物医学研究上具有广阔的应用前景.在双光子荧光成像基础上，实现了适合对厚组织样品观测的背向光学二次谐波成像，探讨了背向二次谐波成像的特点和影响因素.通过对多种生物组织样品进行大量实验，发现胶原纤维和肌肉纤维均可以产生很强的背向二次谐波，并成功地将背向二次谐波成像技术应用于糖尿病患者皮肤的观测.背向二次谐波成像技术可望推广到病理检查等临床应用中.

Backward Second Harmonic Generation Imaging of Tissues

Second harmonic generation (SHG) imaging needs no fluorescent labels and brings no photobleaching or toxicity. It's a promising tool for high-resolution, three-dimensional studies in biomedicine fields. Backward SHG imaging system is established base on a converted laser-scanning two-photon fluorescence microscope. This epifluorescence configuration is especially advantageous to thick tissue imaging and in situ detection. Various tissues were tested to find out those which give rise to strong backward SHG. Then backward SHG imaging was applied in the studies of human skins. The SHG intensity from gangrenosis skins was much lower than that from normal skins. The skins of diabetic patients, which showed no macroscopic abnormality, also displayed a distinct decrease in SHG intensity. The result indicated that collagen in these skins may have suffered from micro-structural disorder or other changes. SHG imaging may serve as a new diagnosis method for dermopathy or other diseases.