DNA as the Intracellular Secondary Target for Antibacterial Action of Human Neutrophil Peptide-I Against Mycobacterium tuberculosis H37Ra |
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Authors: | Sudhir Sharma GK Khuller |
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Institution: | (1) Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India, IN |
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Abstract: | The secondary intracellular target of human neutrophil peptide-1 has been examined in M. tuberculosis H37Ra. Binding studies with radioiodinated HNP-1 revealed biphasic equilibrium binding kinetics with respect to time. The major
site of HNP-1 binding was found to be plasma membrane/cell wall whereas the cytosol appears to be a secondary site. Among
the different macromolecules examined, maximum inhibition (75%) was observed in DNA biosynthesis during treatment with HNP-1.
The interaction of HNP-1 with mycobacterial genomic DNA on the basis of gel retardation assay revealed HNP-1 binding to DNA.
These results indicate that HNP-1 has DNA as the secondary intracellular target for antibacterial action against mycobacteria.
Received: 25 October 2000/Accepted: 10 January 2001 |
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