Interaction of various types of photosystem I complexes with exogenous electron acceptors |
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Authors: | Anastasia A. Petrova Baina K. Boskhomdzhieva Georgy E. Milanovsky Olga A. Koksharova Mahir D. Mamedov Dmitry A. Cherepanov Alexey Yu. Semenov |
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Affiliation: | 1.A.N. Belozersky Institute of Physical-Chemical Biology,Moscow State University,Moscow,Russia;2.Department of Bioengineering and Bioinformatics,Moscow State University,Moscow,Russia;3.A.N. Frumkin Institute of Physical Chemistry and Electrochemistry,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | Interaction of photosystem I (PS I) complexes from cyanobacteria Synechocystis sp. PCC 6803 containing various quinones in the A1-site (phylloquinone PhQ in the wild-type strain (WT), and plastoquinone PQ or 2,3-dichloronaphthoquinone Cl 2 NQ in the menB deletion strain) and different numbers of Fe4S4 clusters (intact WT and FX-core complexes depleted of FA/FB centers) with external acceptors has been studied. The efficiency of interaction was estimated by measuring the light-induced absorption changes at 820 nm due to the reduction of the special pair of chlorophylls (P700 +) by an external acceptor(s). It was shown that externally added Cl 2 NQ is able to effectively accept electrons from the terminal iron-sulfur clusters of PS I. Moreover, the efficiency of Cl 2 NQ as external acceptor was higher than the efficiency of the commonly used artificial electron acceptor, methylviologen (MV) for both the intact WT PS I and for the FX-core complexes. The comparison of the efficiency of MV interaction with different types of PS I complexes revealed gradual decrease in the following order: intact WT?>?menB?>?FX-core. The effect of MV on the recombination kinetics in menB complexes of PS I with Cl 2 NQ in the A1-site differed significantly from all other PS I samples. The obtained effects are considered in terms of kinetic efficiency of electron acceptors in relation to thermodynamic and structural characteristics of PS I complexes. |
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