Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitro

Shoot regeneration was obtained from isolated leaves of Drosera rotundifolia L. cultured on MS media with various concentrations of 6-benzyladenine (BA) and -naphthaleneacetic acid (NAA). The best direct shoot organogenesis was obtained on growth regulator-free medium or medium supplemented with 10^-8 M NAA. Liquid culture medium significantly increased regeneration capacity of leaf tissue. Histological and scanning electron microscopy investigations verify direct plant regeneration without intermediate callus formation. Leaf epidermal cells showed the highest regeneration potential leading to the regeneration of buds. Young shoots with three to seven leaflets rooted spontaneously on the growth regulator-free medium within 38 days of culture and isolated mature plants produced fertile seeds.Abbreviations BA 6-benzyladenine - FAA 40% formalin (5%) +90% acetic acid (5%) +70% ethanol (90%) - ME Murashige and Skoog's (1962) medium - NAA -naphthaleneacetic acid - plumbagin 5-hydroxy-2-methyl-1,4-naphthoquinone - 7-methyljuglone 7-methyl-5-hydroxy-1,4-naphthoquinone - SEM scanning electron microscopy - TEM transmission electron microscopy - PPF photosynthetic photon flux