Chloroplast nitrite uptake is enhanced in Arabidopsis PII mutants |
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Authors: | Ferrario-Méry Sylvie Meyer Christian Hodges Michael |
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Affiliation: | Unité de Nutrition Azotée des Plantes, INRA, Route de St. Cyr, 78026 Versailles Cedex, France. ferrario@versailles.inra.fr |
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Abstract: | In higher plants, the PII protein is a nuclear-encoded plastid protein that regulates the activity of a key enzyme of arginine biosynthesis. We have previously observed that Arabidopsis PII mutants are more sensitive to nitrite toxicity. Using intact chloroplasts isolated from Arabidopsis leaves and (15)N-labelled nitrite we show that a light-dependent nitrite uptake into chloroplasts is increased in PII knock-out mutants when compared to the wild-type. This leads to a higher incorporation of (15)N into ammonium and amino acids in the mutant chloroplasts. However, the uptake differences do not depend on GS/GOGAT activities. Our observations suggest that PII is involved in the regulation of nitrite uptake into higher plant chloroplasts. |
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Keywords: | Col, Colombia ecotype Chl, Chlorophyll DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea MSO, methionine sulfoximine WS, Wassilewskija ecotype |
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