| 重组菌BL21/pET22b-argE固定化条件研究 |
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| 引用本文： | 金圣芳,李环,韦萍.重组菌BL21/pET22b-argE固定化条件研究[J].氨基酸和生物资源,2010,32(3):35-39. |
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| 作者姓名： | 金圣芳 李环 韦萍 |
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| 作者单位： | 南京工业大学生物与制药工程学院,江苏南京,210009;南京工业大学生物与制药工程学院,江苏南京,210009;南京工业大学生物与制药工程学院,江苏南京,210009 |
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| 基金项目： | 国家科技支撑计划重点项目 2008BAI63B07 |
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| 摘 要： | 研究了基因工程菌BL21/pET22b-argE固定化条件。最适包埋材料为海藻酸钙,优化后的包埋条件为海藻酸钠20g.L-1(含有12 g.L-1菌液)滴至2%CaCl2溶液中,固定化16 h。固定化细胞酶拆分蛋氨酸的速率比游离细胞酶慢,但其终极拆分能力与游离细胞酶相当。固定化细胞酶反复利用8批时酶活仍保持在95%以上,具有很好的工业应用优势。
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| 关 键 词： | N-乙酰鸟氨酸脱酰基酶 包埋 海藻酸钙 蛋氨酸 |
Study on Immobilizing Conditions of the Recombinant Strain BL21/pET22b-argE |
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| JIN Sheng-fang,LI Huan,WEI Ping.Study on Immobilizing Conditions of the Recombinant Strain BL21/pET22b-argE[J].Amino Acids & Biotic Resources,2010,32(3):35-39. |
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| Authors: | JIN Sheng-fang LI Huan WEI Ping |
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| Institution: | JIN Sheng-fang,LI Huan,WEI Ping(College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | The immobilizing conditions of the genetic engineering bacteria BL21/pET22b-argE were investigated.The optimum carrier was calcium alginate,and the optimum conditions of embedding were 2% sodium alginate(containing 12 g·L-1 bacteria) in 2% CaCl2 solution,immobilized 16 h.The rate to split methionine was slower than free cell enzyme,but its ultimate capacity was equal to free cell enzyme.The immobilized enzyme was repeatedly used for 8 times to split methionines and the enzyme activity was still about 95%.It has advantage for industrial application. |
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| Keywords: | N-acetyl-L-ornithine deacetylase embedding calcium alginate methionine |
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