Aequorin-based measurements of intracellular Ca2+-signatures in plant cells |
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| Authors: | Axel Mithöfer Christian Mazars |
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| Institution: | 1. Department Biologie I der Ludwig-Maximilians-Universit?t, Botanik, Menzinger Str. 67, D-80638, München, Germany
2. Signaux et Messages Cellulaires chez les Végétaux, UMR CNRS/UPS 5546, 24 Chemin de Borde Rouge, BP 17 Auzeville, F-31326, Castanet-Tolosan, France
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| Abstract: | Due to the involvement of calcium as a main second messenger in the plant signaling pathway, increasing interest has been
focused on the calcium signatures supposed to be involved in the patterning of the specific response associated to a given
stimulus. In order to follow these signatures we described here the practical approach to use the non-invasive method based
on the aequorin technology. Besides reviewing the advantages and disadvantages of this method we report on results showing
the usefulness of aequorin to study the calcium response to biotic (elicitors) and abiotic stimuli (osmotic shocks) in various
compartments of plant cells such as cytosol and nucleus.
Published: December 9, 2002 |
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| Keywords: | Indexing terms" target="_blank">Indexing terms Cell culture Calcium Cytosol |
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