Chromatographic characterization of gastrin/cholecystokinin peptides in bovine and porcine pituitary |
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Authors: | Margery C. Beinfeld |
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Affiliation: | Department of Pharmacology, St. Louis University Medical School 1402 S. Grand Blvd., St. Louis, MO 63104 USA |
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Abstract: | Gastrin/CCK peptides in extracts from bovine and porcine pituitary have been characterized by Sephadex gel filtration, reverse phase liquid chromatography and a CCK radioimmunoassay (RIA) which detects both CCK and gastrin [4]. Porcine pituitary extracts contain a small amount of two peptides with chromatographic behavior similar to porcine antral gastrins. However, bovine pituitaries lack gastrin and contain instead substantial quantities of a peptide which co-elutes with CCK8 sulfate. We have previously shown that rat pituitary also contains CCK8 sulfate-like peptides but lacks gastrin [3]. It is clear from this work that species differences exist in the gastrin/CCK pituitary peptides. This points out the necessity of a careful chemical characterization of pituitary gastrin/CCK peptides in any species prior to physiological or pharmacological experimentation. |
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Keywords: | Gastrin Cholecystokinin Pituitary High pressure liquid chromatography |
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