Enhanced plasmid stability and production of hEGF by immobilized recombinant E. coli JM101 |
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| Institution: | 1. College of Life Science, Zhejiang University (Huajiachi campus), Hangzhou 310029, PR China;2. Department of Chemical Engineering and Bioengineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, PR China;3. Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China;1. Center of Applied Cutaneous Physiology (CCP), Department of Dermatology, Charité-Universitätsmedizin Berlin, Charitéplatz 1 in 10117 Berlin, Germany;2. Beuth University of Applied Sciences Berlin, Luxemburger Straße 10 in 13353 Berlin, Germany;3. UV-Technik, Vogelsbergstr. 27 in 63456 Hanau, Germany;1. Life Sciences Institute, Zhejiang University, Hangzhou 310058, PR China;2. Department of Pharmacy, Xiamen Medical College, Xiamen 361008, PR China |
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| Abstract: | Recombinant Escherichia coli JM101 was immobilized with porous polyurethane foam (PUF) particle as supporter matrix for human epidermal growth factor (hEGF) production. Flask culture showed that cell immobilization in PUF can improve cell growth and hEGF expression. A bubble column and a three-phase fluidized bed bioreactor by self-design was further applied to produce hEGF, respectively. The results demonstrated that PUF is a feasible immobilized supporter material with good biocompatibility. Immobilization could also decrease the probability for segregational plasmid loss and overgrowth of plasmid-free cells. Cell density, plasmid stability and hEGF productivity were higher than those without the foam matrix, respectively. hEGF productivity was enhanced from 8.73 mg/l h of free-culture to 11.4 mg/l h of immobilized cultivation. |
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