Palatinose production by free and Ca-alginate gel immobilized cells of Erwinia sp. |
| |
| Institution: | 1. Department of Mechanical and Electro-Mechanical Engineering, National I-Lan University, I-Lan 260, Taiwan;2. Department of of Mechanical Engineering, Nation Taiwan University of Science and Technolgy, Taipei,10607, Taiwan;3. Deparment of Mechanical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;1. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Xianyang, Shaanxi, China.;2. Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, Shaanxi, China.;1. Department of Food Engineering, Faculty of Engineering, King Mongkut’s University of Technology Thonburi, 126 Pracha-u-Tid Road, Bangmod, Tungkru, Bangkok 10140, Thailand;2. Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, 126 Pracha-u-Tid Road, Bangmod, Tungkru, Bangkok 10140, Thailand |
| |
| Abstract: | Palatinose is a non-cariogenic disaccharide obtained from the enzymatic conversion of sucrose, used in food industries as a sugar substitute. Free and Ca-alginate immobilized cells of Erwinia sp. D12 were used to produce palatinose from sucrose. Palatinose production was studied in a repeated-batch process using different immobilized biocatalysts: whole cells, disrupted cells and glucosyltransferase. Successive batches were treated with the immobilized biocatalyst, but a decrease in palatinose production was observed. A continuous process using a packed-bed reactor was investigated, and found to produce 55–66% of palatinose during 17 days using immobilized cells treated with glutaraldehyde and a substrate flow speed of 0.56 ml min−1. However, immobilized cells in a packed-bed reactor failed to maintain the palatinose production for a prolonged period. The free cells showed a high conversion rate using batch fermentation, obtaining a palatinose yield of 77%. The cells remained viable for 16 cycles with high palatinose yields (65–77%). Free Erwinia sp. D12 cells supported high production levels in repeated-batch operations, and the results showed the potential for repeated reuse. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
