Optimization of cationic polymer-mediated transfection for RNA interference |
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| Authors: | Xiaojie Fan Jingnan Yang Guangyao Wu Meiyi Wang Xiaoxia Cheng Chang Liu Qian Liu Yanan Wen Shuangshuang Meng Zhenxing Wang Xuhong Lin Lei An |
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| Affiliation: | 1.Huaihe Hospital of Henan University, Kaifeng, Henan, China.; 2.Henan University, School of Medicine, Kaifeng, Henan, China. |
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| Abstract: | Transfection efficiency was estimated to optimize the conditions for RNA interference (RNAi), including transfection time, validity, and nucleic acid concentration and type, using the EZ Trans Cell Reagent, a cationic polymer. An shRNA against GFP was designed and transfected into cells using the EZ transfection reagent. The shRNA significantly decreased the expression of GFP. In addition, pre-diluted transfection reagent at room temperature and small nucleic acids increased the transfection efficiency, which peaked at 24 h. Compared with circular nucleic acids, linear nucleic acids showed higher transfection efficiency and a higher genome integration rate. We optimized cationic polymer-mediated RNAi conditions, and our data will be useful for future RNAi studies. |
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| Keywords: | shRNA transfection reagent cationic polymer RNAi. |
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