| RNA干扰VEGF对体外JAR细胞生长的影响 |
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| 引用本文： | 黄向红,伍招娣,谭小军. RNA干扰VEGF对体外JAR细胞生长的影响[J]. 生命科学研究, 2007, 11(1): 72-77 |
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| 作者姓名： | 黄向红 伍招娣 谭小军 |
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| 作者单位： | 湘潭市中心医院,中国湖南,湘潭,411100;中南大学湘雅医院妇产科,中国湖南,长沙,410008;中南大学湘雅医院妇产科,中国湖南,长沙,410008;湘潭市中心医院,中国湖南,湘潭,411100 |
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| 摘 要： | 利用pSUPER质粒作为载体，在人绒癌JAR细胞内成功的沉默了VEGF基因的表达，建立了稳定转染pSUPER—shVEGF1，pSUPER—shVEGF2干扰质粒的JAR细胞株．随后利用RT—PCR证实了在JAR细胞株中，VEGF基因的表达被抑制．显微观察及流式细胞学检测转染后细胞株克隆生长情况显示体外培养条件下VEGF基因受抑后JAR细胞生长周期无发生变化，提示VEGF在体外可能对JAR细胞增殖无直接作用．
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| 关 键 词： | RNA干扰 血管内皮生长因子 JAR细胞 pSUPER载体 |
| 文章编号： | 1007-7847（2007）01-0072-06 |
| 收稿时间： | 2006-08-23 |
| 修稿时间： | 2007-02-06 |
Effect of Stable Suppression of VEGF Gene Expression in JAR Cells |
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| HUANG Xiang-hong,WU Zhao-di,TAN Xiao-jun. Effect of Stable Suppression of VEGF Gene Expression in JAR Cells[J]. Life Science Research, 2007, 11(1): 72-77 |
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| Authors: | HUANG Xiang-hong WU Zhao-di TAN Xiao-jun |
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| Abstract: | RNA interference(RNAi)method was successfully applied in JAR cells with pSUPER vector,targeting to the VEGF gene.The cell lines that the pSUPER-shVEGF vectors were stablly transfected and construced.RT-PCR method was performed to confirm the expression level of VEGF gene in JAR cells.FCM confirmed cell cycles were not changed.It could be concluded that the suppression of expression of VEGF gene by pSUPER system may not influence proliferation of JAR cell. |
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| Keywords: | RNAi VEGF JAR cells pSUPER vector |
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