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The effect of cellular calcium on Na+/K+ cotransport in human red blood cells
Institution:1. Robert F. Smith School for Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA;2. Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14583, USA;1. Dept. of Morphology, Surgery and Experimental Medicine, Section of Pathology, Oncology and Experimental Biology, Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara, Ferrara, Italy;2. Dept. of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland;1. Department of The Central Laboratory, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;2. Department of Pathology, Northern Jiangsu People''s Hospital, Yangzhou, China;3. Department of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;4. Department of Lung, The Third People’s Hospital of Yangzhou, Yangzhou, China;5. Department of Molecular Biology, Inter faculty Institute of Cell Biology, Eberhard Karls University Tuebingen, Tuebingen, Germany
Abstract:The increase in Ca2+ permeability by addition of ionophore A23187 in the presence of external Ca2+ did not alter the bumetanide-sensitive Na+/K+ effluxes in human red blood cells. An inhibition of this pathway by cellular Ca2+ could be observed only under conditions in which the cellular ATP content was drastically depleted.
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