Methanogenesis from trimethylamine + H2 by Methanosarcina barkeri is coupled to ATP formation by a chemiosmotic mechanism |
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| Affiliation: | 1. Department of Energy (DENERG), Politecnico di Torino, Corso Duca Degli Abruzzi, 24, 10129, Turin, Italy;2. Energy Center, Politecnico di Torino, Via Paolo Borsellino 38/16, 10138, Turin, Italy;3. Fondazione Edmund Mach, Biomass and Renewable Energy Unit, Via E. Mach, 1, 38010, San Michele a/A, Italy;4. SOLIDpower Spa, V.le Trento, 115/117, Mezzolombardo, TN, 38017, Italy;1. School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, China;2. Department of Radiation Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA;3. School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA;4. Environmental Engineering Program, Department of Civil Engineering, Auburn University, Auburn, AL 36849, USA;5. Collaborative Innovation Center of Water Quality Safety and Protection in Pearl River Delta, Guangzhou University, Guangzhou 510006, China;1. Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 210011, Tucson, AZ 85721-0011, USA;2. Departamento de Procesos y Tecnología, División de Ciencias Naturales e Ingeniería, Universidad Autónoma Metropolitana – Unidad Cuajimalpa (UAM-C), Vasco de Quiroga 4871, Col. Santa Fe Cuajimalpa, Cuajimalpa de Morelos, C.P. 05300 México, D.F., Mexico;3. Department of Chemistry & Biochemistry, University of Arizona, P.O. Box 210041, Tucson, AZ 85721-0041, USA;4. Department of Soil, Water & Environmental Science, University of Arizona, P.O. Box 210038, Tucson, AZ 85721-0038, USA;1. Institute of Urban Water Management and Environmental Engineering, Ruhr-Universität Bochum, Germany;2. Institute of Geology, Mineralogy and Geophysics, Ruhr-Universität Bochum, Germany |
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| Abstract: | Cell suspensions of Methanosarcina barkeri catalyzed the conversion of trimethylamine and molecular hydrogen to methane according to the equation (CH3)3NH+ + 3 H2 → 3 CH4 + NH+4. The onset of methane formation resulted in an increase of the intracellular ATP content from 2 to 4.6 nmol/mg protein and in the generation of a protonmotive force (Δp) of −130 mV, of which the Δψ contributed 90%. The addition of the uncoupler led to a drastic decrease of the intracellular ATP content and the Δψ, but stimulated methanogenesis. The ATPase inhibitor DCCD caused a rapid exhaustion of the ATP pool and inhibited methane formation, whereas Δψ was not affected. The inhibition of methane formation by DCCD could be relieved by addition of TCS, indicating a chemiosmotic coupling between methane formation according to the above equation and ATP synthesis. |
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