Studies of the spontaneous transfer of retinol from the retinol: retinol-binding protein complex to unilamellar liposomes |
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| Institution: | 1. Division of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand;2. Univ. Lille, Inserm, CHU Lille, U1008, F-59000 Lille, France;1. Pharmacy Discipline, School of Clinical Sciences, Queensland University of Technology, Brisbane, QLD, Australia;2. Institute of Health Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, QLD, Australia;3. School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane, QLD, Australia;4. Translational Research Institute, Brisbane, QLD, Australia;5. Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China;1. Pharmacy Discipline, School of Clinical Sciences, Queensland University of Technology, Brisbane, QLD, Australia;2. Institute of Health Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, QLD, Australia;3. Translational Research Institute, Brisbane, QLD, Australia;4. Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China;1. Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, 110016, Shenyang, Liaoning Province, China;2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, Jiangsu, 222001, China;3. Jiangsu Kanion Parmaceutical CO. LTD, Lianyungang, Jiangsu, 222001, China;1. International University, Ho Chi Minh City, Vietnam;2. Deakin University, Geelong, Australia, School of Medicine;3. College of Pharmacy, Ajou University, Suwon, Republic of Korea;4. Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, Vietnam;5. Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam |
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| Abstract: | The transfer of retinol from its complex with the retinol-binding protein to cell surfaces was studied using unilamellar liposomes as a cell surface model. The transfer of retinol to liposomes at 37°C was rapid and reached an apparent equilibrium within 60 min. The amount of retinol transferred to the liposomes at equilibrium was directly proportional to the starting concentration of retinol: retinol-binding protein over a wide range of retinol:retinol-binding protein concentrations and also directly proportional to the concentration of liposomal phospholipid in the system, when the concentration of retinol:retinol-binding protein was held constant. The transfer increased slightly with temperature. Transfer was increased by a factor of 1.8 at pH 4.5 compared to pH around 7. Prealbumin in amounts sufficient to complex all retinol:retinol-binding protein, decreased retinol transfer to liposomes indicating that prealbumin increases the affinity of retinol-binding protein for retinol. Addition of apo retinol-binding protein to the system decreased the transfer of retinol to liposomes considerably probably through competition with the liposomes for retinol. In similarly designed experiments delipidated bovine serum albumin competed much less with liposomes for retinol. The results show that spontaneous transfer of retinol from the retinol:retinol-binding protein complex to liposomal membranes occurs in vitro and suggests that a similar transfer may occur in vivo from retinol:retinol-binding protein to cell surface membranes. |
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