Polyunsaturated fatty acids are enriched in the plasma membranes of mitogen-stimulated T-lymphocytes |
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| Affiliation: | 1. Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA;2. Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66047, USA;3. Department of Chemistry, The University of Kansas, Lawrence, KS 66047, USA;4. Department of Biological Sciences and Bioengineering Program, Lehigh University, Bethlehem, 18015, USA;5. Biophysics Program, University of Maryland, College Park, MD 20742, USA;1. Division Equine Sciences, Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands;2. Division Cell Biology, Metabolism & Cancer, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands;3. Department of Orthopaedics, University Medical Center Utrecht, Utrecht, Netherlands;4. Division of Infectious Diseases & Immunology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands |
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| Abstract: | Plasma membranes were purified from T-lymphocytes from rabbit thymus stimulated with concanavalin A. Lipids were extracted and the fatty acid composition of the individual phospholipid species was determined by gas-liquid chromatography. Compared to the plasma membranes derived from control cells, the plasma membranes from mitogen-stimulated cells were enriched in polyunsaturated fatty acids. This increase in unsaturation was found in phosphatidylcholine, phosphatidylinositol and phosphatidylserine, while the fatty acid composition of phosphatidylethanolamine was not significantly altered. The phospholipid composition remained almost unchanged during the period of stimulation. The molar ratio cholesterol to phospholipid was decreased. These changes in the lipid composition of plasma membranes from mitogen-stimulated T-lymphocytes are discussed with regard to functional implications. |
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