Binding ability of Clostridium botulinum neurotoxin to the synaptosome upon treatment of various kinds of the enzymes |
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| Institution: | 1. Gilbert Family Neurofibromatosis Institute and Brain Tumor Institute, Children''s National Health System, Washington, DC, USA;2. Department of Pediatric and Adult Neuro-oncology, Centre Léon Bérard and Institute of Pediatric Hematology and Oncology, Lyon, France;1. Department of Surgical Oncology, Shandong Cancer Hospital and Institute, Jinan, China;2. Division of Gynecologic Oncology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA;3. School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, China;4. Department of Gynecologic Oncology, Shandong Cancer Hospital and Institute, Jinan, China;5. Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA;1. Banaras Hindu University, Varanasi, India;2. Veer Bahadur Singh Purvanchal University, Jaunpur, India;3. NIMS University, janupur, India;4. Rajiv Gandhi Proudyogiki Vishwavidyalaya, Bhopal, India |
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| Abstract: | The binding ability of Cl. botulinum neurotoxin to synaptosomes upon treatment with various enzymes (neuraminidase, trypsin, and β-bungarotoxin containing phospholipase A2 activity) was studied. When synaptosomes were treated with neuraminidase, their ability to bind toxin decreased; trypsin and β-bungarotoxin had slightly week or no effect. The decrease in toxin-binding ability of synaptosomes was paralleled by a release of sialic acid from the synaptosomes by the neuraminidase treatment. The toxin-binding ability of synaptosomes treated with neuraminidase was lower than untreated ones at a high concentration of sodium chloride. The binding of the toxin to synaptosomes occurred at least at the two types of structural sites, one site which contained sialic acid, and other site which was sensitive to high ionic strength. It may be possible that another binding state except these is present at the synapse. |
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