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The effects of methyl methanesulphonate on mitosis and chromosomes of excised barley embryos
Authors:T Gichner  M Ondřej  Lim En Se  J Velemínský
Institution:1. Institute of Experimental Botany, Czechoslovak Academy of Scienoes, Flemingovo n. 2, 160 00, Praha, Praha 6, Csechoslovakia
2. Institute of Experimental Biology, Aoademy of Soiences, Pyongyang, Democratic People’s Republic of Korea
Abstract:Excised barley embryos were treated with methyl methanesulphonate and cultivatedin vitro in water or in a nutrient medium. The alkylating compound induced a high frequency of chromosome aberrations observed at anaphase and metaphase and depressed the mitotic division in the root-tips of excised embryos.
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