Factors that affect plant regeneration from in vitro culture of immature seeds in four lentil cultivars |
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Authors: | Polanco María Carmen Ruiz María Luisa |
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Institution: | (1) Area de Genética, Facultad de Biología, Universidad de León, E-24071 León, Spain |
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Abstract: | An efficient and simple method for plant regeneration from immature lentil seeds (Lens culinaris) is described. Immature seeds from 1 to 6 mm of four lentil cultivars were cultured in vitro on 10 different media. Culture media included different concentrations of N
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-benzylaminopurine (BAP), alone or in combination with other phytohormones. After 4 weeks in culture, multiple shoot regeneration
was observed using media with BAP. Immature seed size showed significant effect on shoot regeneration. Regenerated shoots
(up to 4 shoots per explant on medium with Kinetin (KN) and from 5 to 20 on media with BAP) formed adventitious roots 30 days
after transferring them to a medium containing 11.4 μM indole-3-acetic acid (IAA). The efficiency of the rooting medium varied
depending upon the shoot-regeneration medium and the cultivar tested. The highest rooting percentage (88.9%) was obtained
from regenerated shoots of the cultivar Verdina on a medium with 1 μM α-naphthaleneacetic acid (NAA).
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Lens culinaris immature embryo benzylaminopurine indoleacetic acid organogenesis |
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