Determination of linezolid in growth media by high-performance liquid chromatography with on-line extraction |
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Authors: | Ba Boubakar B Nso Branly Bikie Bi Quentin Claudine Saux Marie-Claude |
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Institution: | Pharmacokinetic and Clinical Pharmacy Laboratory, (EA 525), Victor Segalen Bordeaux 2 University, Bordeaux, France. boubakar.ba@phcocin.u-bordeaux2.fr |
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Abstract: | An isocratic high-performance liquid chromatography (HPLC) method with on-line extraction has been developed to determine linezolid in Mueller-Hinton broth. The loading mobile phase consisting of water-acetonitrile 99:1 (v/v) allowed retention of the analyte on a LiChrocart 4-4 pre-column filled with a LiChrospher 100 RP-8, 5 microm. The transfer of the analyte by a backflush mode to a 150 mm x 4.6 mm I.D. Kromasil C8 5 microm column was performed using a mobile phase of water-acetonitrile 80:20 (v/v). UV detection at 254 nm allowed a quantification limit of 0.39 microg/mL with a 50-microL sample size. The method was successfully applied to in vitro pharmacokinetic-pharmacodynamic studies. |
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