| 采用分子生物学技术诊断隐球菌脑膜脑炎的研究 |
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| 引用本文： | 王光裕,陈敏,洪南,王升启,廖万清,杨英. 采用分子生物学技术诊断隐球菌脑膜脑炎的研究[J]. 中国真菌学杂志, 2019, 0(2): 95-98 |
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| 作者姓名： | 王光裕 陈敏 洪南 王升启 廖万清 杨英 |
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| 作者单位： | 军事医学研究院辐射医学研究所;北京市药品检验所;上海市医学真菌分子生物学重点实验室上海长征医院皮肤科 |
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| 基金项目： | 艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2018ZX10101003;2018ZX10712001);中国工程院咨询研究项目(2017-XY-35)资助 |
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| 摘 要： | 目的采用分子检测技术对疑似隐球菌感染的脑膜炎病例进行诊断。方法收集患者的脑脊液样本,提取DNA,设计引物进行PCR扩增,采用DNA芯片技术对扩增产物进行分子检测。结果显示样本新生隐球菌阳性。结论通过ITS保守序列设计引物进行PCR扩增和DNA芯片技术对常规真菌学检查不能确定的疑似隐球菌脑膜炎患者脑脊液样本进行非培养检测,具有实验室诊断参考价值。
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| 关 键 词： | 隐球菌感染 DNA芯片 分子诊断 |
Study on the diagnosis of cryptococcal meningoencephalitis by molecular biological techniques |
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| WANG Guang-yu,CHEN Min,HONG Nan,WANG Sheng-qi,LIAO Wan-qing,YANG Ying. Study on the diagnosis of cryptococcal meningoencephalitis by molecular biological techniques[J]. Chinese JOurnal of Mycology, 2019, 0(2): 95-98 |
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| Authors: | WANG Guang-yu CHEN Min HONG Nan WANG Sheng-qi LIAO Wan-qing YANG Ying |
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| Affiliation: | (Beijing Institute of Radiation Medicine,Beijing 100850;Shanghai Key Laboratory of Molecular Medical Mycology,Department of Dermatology,Changzheng Hospital,Shanghai 200003) |
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| Abstract: | Objective To diagnose the case of meningitis suspected of cryptococcal infection by molecular detection technique.Methods Cerebrospinal fluid samples of patients were collected,DNA was extracted,primers were designed for PCR amplification,and gene detection was performed on the amplified products by DNA chip technology.Results Sample showed positive results for Cryptococcus neoformans.Conclusion PCR amplification of primers designed from ITS conserved sequence and non-culture detection of cerebrospinal fluid samples by DNA chip technology have clinical reference value for deep fungal infections that are difficult to diagnose by traditional methods. |
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| Keywords: | Cryptococcus infection DNA chip molecular diagnosis |
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