Insulin-like growth factor II overexpression does not affect sorting of lysosomal enzymes in NIH-3T3 cells. |
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Authors: | T Braulke R Bresciani D M Buergisser K von Figura |
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Institution: | Georg-August-Universit?t, Biochemie II, G?ttingen, Germany. |
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Abstract: | The sorting of newly synthesized mannose 6-phosphate (M6P)-containing proteins and of the major excreted protein (MEP), a lysosomal thiol proteinase, was studied in NIH-3T3 cells transfected with the cDNA of human insulin-like growth factor II (IGF II) or with the vector alone. Extracts from media and cells labelled with 35S] methionine were used for chromatography on a M6P/IGF II receptor affinity matrix or for immunoprecipitation to assess the distribution of newly synthesized M6P-containing proteins and MEP, respectively. The results indicate that the overexpression of IGF II did not affect the synthesis and the sorting of M6P-containing proteins and of MEP. The binding and uptake of the lysosomal enzyme arylsulfatase A were not affected in IGF II overexpressing cells. |
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