High molecular weight precursors of glucans inSaccharomyces cerevisiae |
| |
| Authors: | José Ruiz-Herrera Germán Larriba |
| |
| Affiliation: | (1) Departments of Genética y Biología Molecular, and Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Gto., México;(2) Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain |
| |
| Abstract: | Nascent  -1,3 glucan synthesized by mixed membrane fractions fromSaccharomyces cerevisiae was solubilized by extraction with hot SDS or urea. Nature of the material was analyzed by electrophoresis and gel filtration. As determined by gel filtration, Mr of synthesized glucans exceeded 1,500 kDa, but was below 20,000 kDa. This nascent material served as an acceptor for further glucose transfer reactions, giving rise to glucan molecules over 20,000 kDa. It is suggested that the high Mr precursor components represent protein-bound glucan molecules in transit to the cell surface. |
| |
| Keywords: | beta glucan glucan synthetase protein acceptor Saccharomyces cerevisiae |
| 本文献已被 SpringerLink 等数据库收录！ |
|
