Application of methods for viral clearance in stem cell production |
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Authors: | Fernando Cobo |
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Institution: | (1) Stem Cell Bank of Andalucía (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas, 2, 18014 Granada, Spain |
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Abstract: | Regenerative medicine therapies will allow in the future the transplant of cells of human origin in some diseases that until
now have been incurable. The assurance of the safety and quality, especially from a microbiological point of view, is very
important for these therapeutic products. Depending on the starting material, there are several sources of pathogen presence,
mainly human viruses. Also, the use of feeders of animal origin as layers in which the stem cells can grow may permit the
transmission of animal pathogens to these cells. However, cell sources are limited due to the low availability of spare in
vitro fecundation human embryos and the low rate of success in the derivation of human stem cell lines. Thus, in several cases,
it will be necessary to evaluate the possibility of removing or inactivating these microorganisms. In this paper, we summarize
the main methods of viral clearance and we have provided an overview of the main features taking into account in the viral
clearance techniques. |
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Keywords: | Viral clearance Stem cell lines Stem cell banks Cell therapy |
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