Faster and easier chromatin immunoprecipitation assay with high sensitivity |
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| Authors: | Kohzaki Hidetsugu Murakami Yota |
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| Affiliation: | Venture Laboratory, Kyoto Institute of Technology, Kyoto, Japan. hkohzaki@virus.kyoto-u.ac.jp |
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| Abstract: | Chromatin immunoprecipitation (ChIP) assays are widely used to investigate where chromatin-binding proteins bind to the genome. The standard assay is very time consuming. We have developed a rapid ChIP assay in which the immunoprecipitates serve directly as PCR templates. This assay eliminates the step to reverse the crosslinking, shortening the assay by 1 day. It also requires a less immunoprecipitating antibody, permits many samples to be tested simultaneously, and is more sensitive than the standard ChIP assay. |
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| Keywords: | ChIP assay (chromatin immunoprecipitation assay) Chromatin binding protein Cross‐linked protein–DNA complex PCR |
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