Extramitochondrial Ca2+ in the Nanomolar Range Regulates Glutamate-Dependent Oxidative Phosphorylation on Demand |
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Authors: | Frank Norbert Gellerich Zemfira Gizatullina Odeta Arandarcikaite Doreen Jerzembek Stefan Vielhaber Enn Seppet Frank Striggow |
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Institution: | 1. KeyNeurotek Pharmaceuticals AG, ZENIT Technology Park, Magdeburg, Germany.; 2. Department of Neurology, Otto von Guericke University Magdeburg, Magdeburg, Germany.; 3. Institute for Biomedical Research, Kaunas University of Medicine, Kaunas, Lithuania.; 4. Department of Pathophysiology, Centre of Molecular and Clinical Medicine, University of Tartu, Tartu, Estonia.;National Institutes of Health, United States of America |
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Abstract: | We present unexpected and novel results revealing that glutamate-dependent oxidative phosphorylation (OXPHOS) of brain mitochondria is exclusively and efficiently activated by extramitochondrial Ca2+ in physiological concentration ranges (S0.5 = 360 nM Ca2+). This regulation was not affected by RR, an inhibitor of the mitochondrial Ca2+ uniporter. Active respiration is regulated by glutamate supply to mitochondria via aralar, a mitochondrial glutamate/aspartate carrier with regulatory Ca2+-binding sites in the mitochondrial intermembrane space providing full access to cytosolic Ca2+. At micromolar concentrations, Ca2+ can also enter the intramitochondrial matrix and activate specific dehydrogenases. However, the latter mechanism is less efficient than extramitochondrial Ca2+ regulation of respiration/OXPHOS via aralar. These results imply a new mode of glutamate-dependent OXPHOS regulation as a demand-driven regulation of mitochondrial function. This regulation involves the mitochondrial glutamate/aspartate carrier aralar which controls mitochondrial substrate supply according to the level of extramitochondrial Ca2+. |
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