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Rapid enumeration of viable Legionella pneumophila serogroup 1
Authors:G Vesey    A Nightingale    D James    D L Hawthorne  J S Colbourne
Institution:Thames Water Utilities plc, New River Head Laboratories, 177 Rosebery Avenue, London EC1R 4TP, UK
Abstract:A rapid assay for the detection of viable Legionella pneumophila serogroup 1 was evaluated. A total of 431 environmental water samples were examined using an immunofluorescent assay (IFA) combined with the cell respiration stain iodonitrotetrazolium violet (INT) and the results compared with conventional culture. The IFA/INT assay was at least as sensitive and much quicker than culture for the detection of viable Legionella pneumophila serogroup 1 in most types of sample.
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