Expressed sequence tags from persimmon at different developmental stages |
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| Authors: | T Nakagawa A Nakatsuka K Yano S Yasugahira R Nakamura N Sun A Itai T Suzuki H Itamura |
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| Institution: | (1) Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue 690-8504, Japan;(2) Faculty of Life and Environmental Sciences, Shimane University, Matsue 690-8504, Japan;(3) Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan;(4) Faculty of Agriculture, Tottori University, Tottori 680-8533, Japan;(5) Graduate School of Science, Nagoya University, Nagoya 464-8601, Japan |
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| Abstract: | Persimmon (Diospyros kaki Thunb.) is an important fruit in Asian countries, where it is eaten as a fresh fruit and is also used for many other purposes.
To understand the molecular mechanism of fruit development and ripening in persimmon, we generated a total of 9,952 expressed
sequence tags (ESTs) from randomly selected clones of two different cDNA libraries. One cDNA library was derived from fruit
of “Saijo” persimmon at an early stage of development, and the other from ripening fruit. These ESTs were clustered into 6,700
non-redundant sequences. Of the 6,700 non-redundant sequences evaluated, the deduced amino acid sequences of 4,356 (65%) showed
significant homology to known proteins, and 2,344 (35%) showed no significant similarity to any known proteins in Arabidopsis databases. We report comparison of genes identified in the two cDNA libraries and describe some putative genes involved in
proanthocyanidin and carotenoid synthesis. This study provides the first global overview of a set of genes that are expressed
during fruit development and ripening in persimmon. |
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| Keywords: | Carotenoid Diospyros kaki Fruit Proanthocyanidin Ripening |
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