The identification and quantification of three new 6alpha-hydroxylated corticosteroids in human neonatal urine |
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Authors: | H J Derks N M Drayer |
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Affiliation: | Department of Pediatrics, University Hospital, Groningen, The Netherlands |
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Abstract: | The 24 hours urines of six two days old fullterm newborn infants were investigated for polar corticosteroids. 6alpha-hydroxy-tetrahydrocortisone, 6alpha-hydroxy-20alpha-cortolone and 6alpha-hydroxy-20beta-cortolone were identified by gas chromatographic-mass spectrometric comparison of the urinary steroids to compounds synthesized previously. These 6alpha-hydroxylated corticosteroids as well as seven other polar corticosteroids were quantified by gas chromatography or mass fragmentography. It was shown that the newly identified steroids constituted a quantitatively important part of the neonatal urinary corticosteroids. The unconjugated- and glucuronic acid conjugated steroids were quantified separately. It was found that the extent of glucuronoconjugation decreased with increasing polarity of the steroid moiety. |
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Keywords: | MO-TMS O-methoxime-trimethylsilyl ether (persilylated) TMS trimethylsilyl ether (persilylated) GC-MS combined gas chromatography-mass spectrometry HPLC high performance liquid chromatography m/e mass-charge ratio molecular ion cortisone 17α,21-dihydroxy-pregn-4-ene-3,11,20-trione Cortisol 11β,17α,21-trihydroxy-pregn-4-ene-3,20-dione (A)THE (allo)tetrahydrocortisone 3α,17α,21-trihydroxy-5α/β-pregnane-11,20-dione (A)THF (allo)tetrahydrocortisol3α,11β,17α,21-tetrahydroxy5α/β-pregnane-20-one 6β-OHF 6β-hydroxy-cortisol 6β,11β,17α,21-tetrahydroxy-pregn-4-ene-3,20-dione 6β-OHE 6β-hydroxy-cortisone 6β,17α,21-trihydroxy-pregn-4-ene-3,11,20-trione α/β-cortolone 3α,17α,20α/β,21-tetrahydroxy-5β-pregnane-11-one α/β-cortol 3α,11β,17α,20α/β,21-pentahydroxy-5β-pregnane 6α-OH(A)THE 6α-hydroxy-(allo)tetrahydrocortisone 3α,6α,17α,21-tetrahydroxy-5α/β-pregnane-11 20-dione 6β-OH(A)THE 6β-hydroxy-(allo)tetrahydrocortisone 3α,6β,17a,21-tetrahydroxy-5α/β-pregnane-11,20-dione 6α-OH(A)THF 6α-hydroxy-(allo)tetrahydrocortisol 3α,6α,11β,17α,21-pentahydroxy-5α/β-pregnane-20-one 6β-OH(A)THF 6β-hydroxy-(allo)tetrahydrocortisol 3α,6β,11β,17α,21-pentahydroxy-5α/β-pregnane-20-one 6α-OH-α/β-cortolone 6α-hydroxy-20α/β-cortolone 3α,6α,17α,20α/β,21-pentahydroxy-5β-pregnane-11-one 1β-hydroxy-20β-cortolone 1β,3a,17a,20β,21-pentahydroxy-5β-pregnane-11-one methyl deoxycholate 3α,12α-dihydroxy-5β-cholanic acid methyl ester |
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