利用S位点多态性快速测定甘蓝自交不亲和性及其S等位基因系

Rapid Identification of Self-incompatibility and S-alleles of Cabbage by the Polymorphism of S Locus

Specific primers were designed according to the sequences of class Ⅰ and class Ⅱ SLG genes. The PCR products using these primers amplified from the cabbage　(Brassica oleracea L.) gDNAs of Southwest China Agricultural University (SCAU) and Horticulture Research International (HRI), Wellesbourne, showed that those with class Ⅰ SLG genes were strong self-incompatibility (SI) lines and those with class Ⅱ were included both strong and weak SI lines. Thus the whole length of DNA fragment of class Ⅱ SLG gene may be the molecular marker for distinguishing SI lines from SC lines. Furthermore, RFLP analysis of the class 　Ⅱ SLG genes from various S-alleles of cabbage was conducted by using 6 restriction endonucleases which recognize 4 bp DNA sequence and the results showed that the S-alleles from HRI as well as the weak SI lines from SCAU presented obvious different RFLP profiles which could be used for distinguishing S-alleles of cabbage.