利用RAPD标记构建美洲黑杨×欧美分子标记连锁图谱 |
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作者姓名: | 张新叶 尹佟明 诸葛强 黄敏仁 朱立煌 翟文学 邬荣领 王明庥 |
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作者单位: | 1.南京林业大学林木遗传和基因工程国家林业局重点实验室,南京 2100372.中国科学院遗传研究所植物生物技术重点实验室,北京 1001013.North Carolina State University,Ralegh,NC 98195 USA1.Nanjing Forestry University,Nanjing 210037,China2.nstitute of Genetics,Chinese Academy of sciences,Beijing 100101,China3.North Carolina State University,Ralegh,NC 98195 USA |
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基金项目: | 国家高技术研究发展计划(863计划) |
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摘 要: | 本文利用RAPD标记和美洲黑杨(Populus deltoides)×欧美杨(P.euramericana)的F1群体,构建了美洲黑杨×欧美杨的分子标记连锁图谱。实验过程中对1040个寡核苷酸随机引物进行了重复筛选,共选出127个引物用于作图群体(包括双亲共92个无性系)的随机扩增,这127个引物产生229个多态基因座,其中符合“拟测交”1∶1分离的有214个。利用多点连锁分析,形成19个连锁群及6个三连体和14个连锁对。由19个连锁群构成的图谱含标记129个,总图距为1914.2cM,覆盖杨树基因组约73.62%。标记间的平均间距为14.84cM。本研究获得了中等密度的美洲黑杨×欧美杨的一个连锁框架。Abstract:A molecular linkage map was constructed for the parents of a P.deltoides × P.euramericana F1 family based on random amplified polymorphic DNA(RAPD)markers.A set of 1040 random oligonucleotide primers were screened and 127 primers were selected to generate RAPD markers within a sample of 90 F1 progenies.A total of 229 segregating loci were identified.Among the 229 loci,15 loci were found distorted from the normal 1∶1 ratio.Using multiple analysis,the 214 markers formed 19 main Linkage groups (including 129 markers)and b triples and 14 pairs.The resulting Linkage map of Populus deltoides × P.euramericana (including 129 markers)spanned 1914.2cM(73.62% coverage of genome length)with an average distance of 14.84cM between markers.
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关 键 词: | 美洲黑杨×欧美杨 Key words molecular linkage map RAPD RAPD 分子标记连锁图谱 |
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