Composition and immunochemical characteristics of exopolysaccharides from the rhizobacterium <Emphasis Type="Italic">Paenibacillus polymyxa</Emphasis> 1465 |
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| Authors: | I V Yegorenkova K V Tregubova L Yu Matora G L Burygin V V Ignatov |
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| Institution: | (1) Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049, Russia |
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| Abstract: | Exopolysaccharides (EPS) synthesized by Paenibacillus polymyxa 1465 in the course of batch cultivation were proven to contain neutral and acidic fractions. EPS are heterogeneous polysaccharides, represented by a complex of macromolecules with molecular mass of 7 × 104 to 2 × 106 Da. The acidic component was shown to be predominant in EPS preparations isolated from bacteria cultivated on glucose, which corresponds to a higher viscosity of EPS water solutions. The exoglycans were shown to contain glucose, mannose, galactose, and uronic acids. Polyclonal rabbit antibodies against the isolated P. polymyxa 1465 EPS preparations were used in a comparative immunodiffusion analysis of a number of P. polymyxa strains. |
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| Keywords: | Paenibacillus polymyxa exopolysaccharides viscosity monosaccharide composition immunodiffusion analysis |
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